FAQ ndeyeMhuka Yese RNA Isolation kit

Ongororo inotevera yezvinetso zvaungasangana nazvomhuka mhuka / sero RNA kutorwa will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

RNA haina kubviswa kana RNA goho rakaderera

Panowanzove nemhando dzakasiyana dzezvinhu zvinokanganisa kudzoreredza kushanda zvakanaka, senge: tishu sampuli RNA yemukati, nzira yekushanda, elution vhoriyamu, nezvimwe.

1. Ice bath kana cryogenic (4 ° C) centrifugation yakaitwa panguva yekushanda.

Kurudziro: Shanda pamhepo tembiricha (15-25 ° C) mukati mese muitiro, usaite ice yekugezesa uye centrifuge pane yakaderera tembiricha.

2. Kuchengetedzwa kwemuenzaniso kusina kukodzera kana nguva yakawandisa yekuchengetedza sampuli.

Kurudziro: Chengetedza samples pa -80 °C kana kuomesa munitrogen yemvura uye dzivirira kudzokororwa kushandiswa kwechando-nyoro;edza kushandisa matishu matsva kana maseru akagadzirwa eRNA kutorwa.

3. Kusakwana sampuli lysis.

Kurudziro: Paunenge uchigadzira matishu, ita shuwa kuti matishu akanyatsoenderana uye kuti masero ematishu akapatsanurwa zvakakwana kutsanangura kuburitswa kweRNA.

4. Iyo enzi haina kuwedzerwa nemazvo.

Kurudziro: Simbisa kuti RNase-Yemahara ddH₂O inowedzerwa kudonha kusvika pakati peyekucheneswa column membrane.

5. Vhoriyamu chaiyo yeabsolute ethanol haina kuwedzerwa kuBuffer RL2 kana Buffer RW2.

Kurudziro: Tevedzera mirairo, wedzera iyo chaiyo vhoriyamu yeabsolute ethanol kuBuffer RL2 uye Buffer RW2 uye sanganisa zvakanaka usati washandisa kiti.

6. Tissue sample dosage haina kukodzera.

Kurudziro: Shandisa 10-20 mg yetishu kana (1-5) × 10⁶masero pa 500 μl buffer RL1, sezvo kushandiswa kwematishu zvakanyanya kunogona kukonzera kuderedzwa kweRNA.

7. Vhoriyamu yakashata isina kunaka kana kuti kuburitsa kusina kukwana.

Kurudziro: Vhoriyamu yekujekesa yekoramu yekuchenesa ndeye 50-200 μl;kana iyo elution mhedzisiro isingagutsi, zvinokurudzirwa kuwedzera tembiricha yekuisa yekamuri nguva mushure mekuwedzera preheated RNase-Mahara ddH.₂O, eg kwe5-10 min.

8.Koramu yekuchenesa ine ethanol yakasara mushure meBuffer RW2 wash.

Kurudziro: Kana paine ethanol yakasara mushure mekugezwa kweBuffer RW2, isina chinhu chubhu centrifugation ye1min, nguva yekushayikwa kwechubhu centrifugation operation inogona kuwedzerwa kusvika 2min, kana mbiru yekuchenesa inogona kuiswa pakushambidzika kwemaminetsi mashanu kuti ibvise zvakakwana ethanol yakasara.

Yakanatswa RNA yakaderedzwa

Hunhu hweRNA yakacheneswa hune hukama nezvinhu zvakaita sekuchengetedza sampuli, kusvibiswa kweRNase, uye kunyengera, nezvimwe.

1. Tissue samples haisi kuchengetwa munguva.

Kurudziro: Kana masamples ematishu kana maseru akasashandiswa nenguva mushure mekuunganidzwa, pakarepo cryopreserve pa -80 ° C kana liquid nitrogen.Kuti ubvise RNA, shandisa ichangobva kutorwa tishu kana sero sampuli pese pazvinogoneka.

2. Kudzokororwa kwechando-kunyunguduka kwemasampuli ematishu.

Kurudziro: Paunenge uchichengeta matishu samples, zviri nani kuacheka kuita zvidimbu zvidiki kuti zvichengetedzwe, uye kubvisa chimwe chezvidimbu paunenge uchizvishandisa kudzivirira kudzokororwa nechando-kunyunguduka kwesample uye kushatiswa kweRNA.

3. RNase inounzwa kana kusapfeka magirovhosi anoraswa, masiki, nezvimwewo panguva yekushanda.

Kurudziro: RNA yekuwedzera yekuedza inoitwa zvakanyanya mumakamuri akasiyana eRNA manipulation uye tafura inocheneswa isati yaedzwa.

Pfeka magurovhosi anoraswa uye masks panguva yekuyedza kuderedza kuderedzwa kweRNA kunokonzerwa nekuunzwa kweRNase.

4. Zvigadzirwa zvakasvibiswa neRNase panguva yekushandiswa.

Kurudziro: Tsiva neMhuka Yakazara RNA Isolation Kit yezviyedzo zvinoenderana.

5. Machubhu ecentrifuge, mazano, nezvimwewo zvinoshandiswa muRNA manipulation zvakasvibiswa neRNase.

Kurudziro: Simbisa kuti machubhu ecentrifuge, matipi, mapipette, nezvimwewo anoshandiswa muRNA kudhirowa ese RNase-Mahara.

Yakacheneswa yakawanikwa RNA inokanganisa kuyedza kwepasi

RNA yakacheneswa nekoramu yekuchenesa, kana maion emunyu, mapuroteni akawandisa anozokanganisa kuyedza kwakadzika, senge: reverse transcription,Northern Blot et al.

1. Iyo elutioned RNA ine salt ion residues.

Kurudziro: Simbisa kuti vhoriyamu chaiyo ye ethanol yawedzerwa kuBuffer RW2 uye ita 2 mbiru yekunatsa washes pa centrifugal speed inoratidzwa kuti ishande;kana paine ion inosara yemunyu, siya mbiru yekuchenesa kuBuffer RW2 kwemaminetsi mashanu patembiricha yekamuri uye ita centrifugation kuti uwedzere kubviswa kwemunyu.

2. Ethanol yakasara mune elutioned RNA.

Kurudziro: Simbisa kuti mushure mekutsvaira RW2 kushambidza, ita isina chinhu tube centrifugation operation pa centrifugation speed inoratidzwa kuti ishande, wedzera nguva yekushanda isina chinhu chubhu centrifugation kusvika 2 min kana ichine ethanol yakasara, kana kuisiya pane imwe tembiricha ye 5 min mushure mokunge isina chinhu chubhu centrifugation kuti iwedzere kubviswa kwe ethanol.

Kuparadzaniswa kweiyo nulceic acid sampuli mhando

Foregene's RNA Isolation kits inogona kuwana yakazara RNA kuparadzaniswa / kubviswa kubva kune anotevera emuenzaniso souces: mhuka yemhuka, chirimwa, maseru, hutachiona, ropa, nezvimwe.

Ndochengeta sei kit

Kuchengetera tembiricha yemumba kwemwedzi makumi maviri nemana.