Kugadzirwa kweinjini yeutsi kwakaunza shanduko yemaindasitiri, kufambira mberi kwemakomputa kwakagadzira nyika yazvino yedhijitari, uye shanduko yetekinoroji ichaunza kufambira mberi kwenguva!Kuberekwa kwaSero Yakananga RT-qPCR KityeForegene yave kuda kuunza nguva nyowani yekuonekwa kwema molecular!

7min Tora RNA template

Ehe, wakazvinzwa zvakanaka, usakahadzika, kubva muchitokisi kuenda kuRT-qPCR, ikozvino nhanho mbiri chete

Stop Reaction

Q:Kutorwa kweRNA kwakakwana pakumhanya kwakadaro?Pachava nekusvibiswa kweDNA?

A: Usanetseke, zvigadzirwa zvedu zvakaenzaniswa zvisingaverengeke uye ongororo, uye hapana mutsauko kana uchienzaniswa nemhedzisiro yezvechinyakare RT-qPCR kuyedza.Uyezve, saizi yemuenzaniso idiki, uye kuyedza kunogona kuitwa nemaseru gumi ane tsika.Ingave iri 6, 12, 24 kana 96-tsime ndiro, masero anogona lysed zvakananga mumatsime eplate, iyo iri nyore uye inokurumidza.Iine 5 × Direct RT Mix uye 2 × Direct qPCR Mix muchigadzirwa, RNA template inogona kushandiswa kwepamusoro-soro reversal uye yakananga amplification, nekunzwa kwepamusoro, kusimba kwakasimba uye kugadzikana kwakanaka.The reverse transcriptase qPCR kit iri nyore kwazvo.

Sero Yakananga RT-qPCR Kitkwete chete ine yepamusoro lysis system, asiwo ine kurwisana kwakasimba kune RT-qPCR reaction inhibitors, iyo inoita kuti kiti yose ive nemuganhu wakaderera wekuona, uye inogona kuramba ichiwana chigumisiro chakanaka pamasero gumi.

Ikozvino kune SYBR yegirini qPCR uye Taqman qPCR nzira dziripo.