Muchikamu chekutanga chekubuda, nekuda kwekukurumidza kukura, kukurumidza kuongororwa kwevanofungidzirwa varwere ndiyo kiyi yekudzivirira COVID-19.Mamwe akatenderwa nucleic acid yekuona reagents ane nguva pfupi yekuvandudza, uye kune matambudziko akadai sekukurumidza kuita simbiso, kusakwana reagent optimization, uye misiyano mikuru pakati pemabhechi;Matambudziko emarabhoritari akasiyana ekiriniki mune akasiyana siyana eiyo nucleic acid yekuona maitiro anogona zvakare kukanganisa iko kurongeka kweiyo nucleic acid yekuona mhedzisiro.Chinyorwa ichi chinotarisa pane akakosha malink uye mapoinzi mune yazvino SARS-CoV-2 nucleic acid yekuona, uye kuongorora matambudziko ekunyepa kwakashata uye kwakanaka kuongororazve kwerabhoritari nucleic acid yekuonekwa uye kusawirirana kwekiriniki.

Nheyo dzeSARS-CoV-2 nucleic acid yekuona

SARS-CoV-2 hutachiona hweRNA hune genome sequence inosvika makumi maviri nepfumbamwe kb, ine gumi majini, anogona kunyatso encode gumi mapuroteni.Mavhairasi anoumbwa neRNA neprotein, uye iyo yekunze yakaturikidzana ndeyekunze yakavharwa inoumbwa nelipids uye glycoprotein.Mukati, puroteni capsid inoputira iyo RNA mairi, nekudaro ichidzivirira iyo iri nyore kuora RNA (P1).

P1 Chimiro cheSARS-COV-2

Mavhairasi anopinda mumasero kuburikidza nechaiyo cell surface receptors kuti akonzerese hutachiona, uye anoshandisa maseru anogashira kutevedzera.

Nheyo yehutachiona nucleic acid yekuona ndeyekuburitsa hutachiona hweRNA kuburikidza nesero lysate, uyezve kushandisa chaiyo-nguva fluorescent reverse transcription-polymerase chain reaction (RT-PCR) kuti ionekwe.

Kiyi yemusimboti wekuona ndeye kushandisa maprimers uye maprobes kuti uwane "kwakatariswa kufananidzwa" kweiyo nucleic acid sequences, kureva, kutsvaga iyo nucleic acid sequence yeSARS-CoV-2 iyo yakasiyana nemamwe mavhairasi mune angangoita zviuru makumi matatu mabhesi (kufanana kwenucleic acid kune mamwe mavhairasi) "Yakaderera" nzvimbo), madhizaini uye probes.

Iwo maprimers uye probes anoenderana zvakanyanya neiyo chaiyo dunhu reSARS-CoV-2 nucleic acid, ndiko kuti, iyo chaiyo yakasimba.Kana iyo chaiyo-nguva fluorescent RT-PCR yekukwidziridza mhedzisiro yesampuli kuti iongororwe yakanaka, inoratidza kuti SARS-CoV-2 iripo mumuenzaniso.Ona P2.

P2 Matanho eSARS-CoV-2 nucleic acid kutsunga (chaiyo-nguva fluorescent RT-PCR)

Mamiriro uye zvinodiwa murabhoritari yeSARS-CoV-2 nucleic acid yekuona

Nucleic acid yekuyedza marabhoritari ndiyo yakanyanya kunaka kune isina kunaka nharaunda yekumanikidza, uye ivo vanofanirwa kutarisisa kudzvanywa kwekutarisa, kuchengetedza mhepo kuyerera, uye kubvisa aerosols.Nucleic acid yekuyedza vashandi vanofanirwa kuve neanoenderana magwaro, kugamuchira yakakodzera polymerase chain reaction kudzidziswa uye kupasa ongororo.Murabhoritari inofanirwa kutariswa zvakanyanya, yaiswa panzvimbo, uye vashandi vasina basa vanorambidzwa zvachose kupinda.Nzvimbo yakachena inofanira kufefeterwa uye kuuraya utachiona panzvimbo.Zvinhu zvakakodzera zvinoiswa munzvimbo, dzakachena uye dzakasviba dzinoparadzaniswa, dzinotsiviwa nenguva, uye dzakasvibiswa munzvimbo.Routine disinfection: Chlorine-containing disinfectant ndiyo mhinduro huru yenzvimbo dzakakura, uye 75% doro rinogona kushandiswa kunzvimbo diki.Nzira yakanaka yekubata neaerosols ndeyekuvhura mahwindo ekupinza mhepo, uye disinfection yemhepo inogona zvakare kuitwa nemirairo yeultraviolet, kusefa, uye disinfection yemhepo.

Makiyi ekubatanidza uye ma paramita eSARS-CoV-2 nucleic acid kutsunga (chaiyo-nguva fluorescent RT-PCR)

Kunyangwe marabhoritari achiwanzo nyatso kutarisisa kune nucleic acid "kuonekwa", kutaura zvazviri, nucleic acid "kubvisa" ndeimwe yematanho akakosha ekuonekwa kwakabudirira, ayo ane hukama zvakanyanya nekuunganidzwa uye kuchengetwa kwemasampuli ehutachiona.

Parizvino, iyo inonyanya kushandiswa yekufema samples, senge nasopharyngeal swabs, shandisa nzira yechipiri, inova isinactivation (kuchengetedza) mhinduro yakagadzirirwa zvichienderana neiyo nucleic acid yekubvisa uye lysis mhinduro.Kune rimwe divi, iyi hutachiona hwekuchengetedza mhinduro inogona kudhiza puroteni yehutachiona, kurasikirwa nebasa rayo uye kusisatapukirwe, uye kunatsiridza kuchengetedzwa kwenzvimbo yekufambisa uye yekuona;kune rumwe rutivi, inogona kutsemura hutachiona zvakananga kuburitsa nucleic acid, kubvisa iyo nucleic acid inoora enzyme, uye kudzivirira hutachiona.Iyo RNA yakaderedzwa.

A virus sampling solution yakagadzirirwa pahwaro hwenucleic acid extraction lysis solution.Zvikuru zvinoumba munyu wakadzikama, ethylenediaminetetraacetic acid chelating agent, guanidine munyu (guanidine isothiocyanate, guanidine hydrochloride, nezvimwewo), anionic surfactant (dodecane) Sodium sulfate), cationic surfactant (tetradecyl methyloxalate, hydrochloride, hydrochloride, hydrochloride), proteinase K uye zvimwe zvakati wandei kana zvimwe zvikamu.Parizvino, kune akawanda marudzi eiyo nucleic acid yekubvisa kits, uye akasiyana nucleic acid kutorwa uye kucheneswa reagents anoshandiswa.Kunyangwe kana iyo imwecheteyo nucleic acid yekubvisa uye yekuchenesa reagent yakashandiswa, maitiro ekubvisa ega ega kiti akasiyana.

Parizvino, iyo nucleic acid yekuona kit zvigadzirwa zvakabvumidzwa neNational Medical Products Administration zvinosarudzwa zvichibva paORF1ab, E uye N genome muSARS-CoV-2 genome.Misimboti yekuona yezvigadzirwa zvakasiyana zvakangofanana, asi maprimers uye probe magadzirirwo akasiyana.Kune zvikamu-zvinangwa zvimwechete (ORF1ab), zvikamu zviviri-zvakanangwa (ORF1ab, N kana E), uye zvikamu zvitatu-zvinangwa (ORF1ab, N uye E).Musiyano uripo pakati pekuonekwa nekududzirwa, nucleic acid kudhirowa uye chaiyo-nguva fluorescent RT-PCR reaction system inofanirwa kureva kune yakakodzera kit mirairo, uye zvinokurudzirwa kuti vashandisi vanyatsotevedzera kududzira nzira inotsanangurwa mukiti mirairo yekududzira.Matunhu akajairwa, maprimers uye probe sequence anokwidziridzwa nechaiyo-nguva fluorescent RT-PCR inoratidzwa muP3.

P3 Nzvimbo yeSARS-CoV-2 amplicon inotariswa pane genome uye kutevedzana kwemaprimers uye probes.

Dudziro yemhedzisiro yeSARS-CoV-2 nucleic acid kutsunga (Rhora-Tine fluorescent RT-PCR)

"Pneumonia Prevention and Control Plan yeSARS-CoV-2 Infection (Second Edition)" kekutanga yakajekesa maitiro ekutonga mhedzisiro yekukwidziridzwa kwegeneti rimwe chete:

1. Hapana Ct kana Ct≥40 isina kunaka;

2. Ct <37 yakanaka;

3. Iko Ct kukosha kwe37-40 ndiyo nzvimbo yegrey-scale.Inokurudzirwa kudzokorora kuedza.Kana mhedzisiro yekudzokorora Ct <40 uye curve yekusimudzira iine mapikisi ari pachena, sampu inotongwa seyakanaka, zvikasadaro haina kunaka.

Chinyorwa chechitatu chegwaro uye chinyorwa chechina chegwaro rakaenderera nemaitiro ari pamusoro.Zvisinei, nekuda kwezvinangwa zvakasiyana-siyana zvinoshandiswa mumakiti ekutengeserana, iyo yambotaurwa ye3rd edition yegwaro haina kupa maitiro ekugadzirisa kusanganiswa kwezvinangwa, kusimbisa Mirayiridzo inopiwa nemugadziri ichakunda.Kutanga kubva pachinyorwa chechishanu chemirayiridzo, zvinangwa zviviri zvakajekeswa, kunyanya maitiro ekutonga kwechinangwa chimwe chete chakaoma kutonga.Ndokunge, kana marabhoritari ichida kusimbisa kuti nyaya yakanaka yeSARS-CoV-2 nucleic acid yekuona, zvinotevera zvinoda kusangana 1 pamamiriro maviri:

(1) Zvinangwa zviviri zveSARS-CoV-2 (ORF1ab, N) mumuenzaniso mumwe chete zvinoongororwa zvakanaka nechaiyo-nguva fluorescent RT-PCR.Kana chinangwa chimwe chete chiri chakanaka, kuongorora zvakare uye kuongorora zvakare kunodiwa.Kana mhedzisiro yebvunzo iri Kana chinangwa chimwe chete chichiri chakanaka, chinotorwa sechakanaka.

(2) Mienzaniso miviri ye-real-time fluorescent RT-PCR yakaratidza chinangwa chimwe chete chakanaka panguva imwe chete kana sampuli mbiri dzemhando imwechete dzakaratidza chinangwa chimwe chete chakanaka chekuedza, chinogona kutongwa sechakanaka.Nekudaro, iyo nhungamiro inosimbisawo kuti mhedzisiro yakaipa yekuongororwa kwenucleic acid haigone kusarura hutachiona hweSARS-CoV-2.Zvinhu zvinogona kukonzera manyepo enhema zvinoda kubviswa, zvinosanganisira hurombo hwemhando yemhando (yekufema samples kubva kune oropharynx uye zvimwe zvikamu), sampuli yekuunganidza yakakurumidza kana kunonoka, Samples haina kuchengetwa, kutakurwa, uye kugadziriswa nemazvo, uye tekinoroji pachayo yaive nematambudziko (virus variation, PCR inhibition), nezvimwe.

Zvikonzero zvekunyepa zvisina kunaka mukuonekwa kweSARS-CoV-2

Pfungwa ye "nhema isina kunaka" mukuyedzwa kweiyo nucleic acid iyo iripo parizvino, inowanzo reva "nhema dzisina kunaka" umo iyo nucleic acid bvunzo mhinduro isingaenderane nekuratidzwa kwekiriniki, ndiko kuti, zviratidzo zvekiriniki uye mhedzisiro yekufungidzira zvinofungidzirwa zvakanyanya nezveCCIDID-19, asi nucleic acid bvunzo dzinogara "dzisina kunaka" kakawanda.Clinical Laboratory Center yeNational Health Commission yakatsanangura "nhema dzisina kunaka" SARS-CoV-2 bvunzo.

(1) Pane chitsama chakati choutachiona mumasero omunhu ane utachiona.Dhiyabhorosi iripo inoratidza kuti mushure mokunge muviri wabatwa nehutachiona, hutachiona hunopinda muhuro kuburikidza nemhino nemuromo, zvino kune trachea uye bronchi, uye hunosvika kune alveoli.Munhu ane hutachiwana achaona nguva yekudyara, zviratidzo zvinyoro, uyezve maitiro ezviratidzo zvakakomba, uye matanho akasiyana echirwere.Uye huwandu hwehutachiona huripo munzvimbo dzakasiyana dzemuviri hwakasiyana.

Maererano nehuwandu hwehutachiwana hwemhando dzemasero, alveolar epithelial masero (pasi rekufema turakiti)> airway epithelial cells (kumusoro kwekufema turakiti)> fibroblasts, endothelial cells, uye macrophages, nezvimwewo;kubva kurudzi rwemuenzaniso, alveolar lavage fluid (yakanyanya Kunyanya kunaka)>kukosora kwakadzika sputum>nasopharyngeal swab>oropharyngeal swab>ropa.Mukuwedzera, utachiona hunogonawo kuonekwa mutsvina.Zvisinei, tichifunga nezvekureruka kweoparesheni uye kugamuchirwa kwevarwere, chirongwa chekliniki chinowanzo shandiswa ioropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (kuvhiya kwakaoma) uye sputum yakadzika (kazhinji chikosoro chakaoma, chinonetsa kuwana).

Nokudaro, huwandu hwehutachiona mumasero eoropharynx kana nasopharynx yevamwe varwere hudiki kana hushoma.Kana chete samples dzeoropharynx kana nasopharynx dzakatorwa kuongororwa, iyo viral nucleic acid haizoonekwa.

(2) Hapana masero ane hutachiona akaunganidzwa panguva yekuunganidza sampuli, kana viral nucleic acid haina kuchengetedzwa zvakanaka.

[① Saiti yekuunganidza isina kunaka, semuenzaniso, pakuunganidza oropharyngeal swabs, kudzika kwekuunganidza hakuna kukwana, iyo yakaunganidzwa nasopharyngeal swabs haina kuunganidzwa yakadzika mumhino yemhino, nezvimwewo. Mazhinji masero akaunganidzwa anogona kunge asina hutachiona masero;

②Sampling swabs inoshandiswa zvisiri izvo.Semuenzaniso, mafiber ekugadzira akadai sePE fiber, polyester fiber uye polypropylene fiber anokurudzirwa kune izvo zvemusoro we swab.Mafibhi echisikigo akadai sekotoni anoshandiswa mukushanda chaiko (yakasimba adsorption yeprotein uye isiri nyore kugeza kunze) Uye nylon fibers (yakashata kunyura kwemvura, zvichiita kuti kusakwana sampling volume);

③Kusashandiswa kwakashata kwemachubhu ekuchengetera hutachiona, sekushandisa zvisizvo kwepolypropylene kana polyethylene machubhu ekuchengetera epurasitiki ari nyore kutora nucleic acids (DNA/RNA), zvichikonzera kudzikira kwehuwandu hwenyucleic acid mumhinduro yekuchengetedza.Mukuita, zvinokurudzirwa kushandisa polyethylene-propylene polymer plastiki uye mamwe akanyatsogadziriswa polypropylene mapurasitiki emidziyo kuchengetedza hutachiona nucleic acids.]

[① Saiti yekuunganidza isina kunaka, semuenzaniso, pakuunganidza oropharyngeal swabs, kudzika kwekuunganidza hakuna kukwana, iyo yakaunganidzwa nasopharyngeal swabs haina kuunganidzwa yakadzika mumhino yemhino, nezvimwewo. Mazhinji masero akaunganidzwa anogona kunge asina hutachiona masero;

②Sampling swabs inoshandiswa zvisiri izvo.Semuenzaniso, mafiber ekugadzira akadai sePE fiber, polyester fiber uye polypropylene fiber anokurudzirwa kune izvo zvemusoro we swab.Mafibhi echisikigo akadai sekotoni anoshandiswa mukushanda chaiko (yakasimba adsorption yeprotein uye isiri nyore kugeza kunze) Uye nylon fibers (yakashata kunyura kwemvura, zvichiita kuti kusakwana sampling volume);

③Kusashandiswa kwakashata kwemachubhu ekuchengetera hutachiona, sekushandisa zvisizvo kwepolypropylene kana polyethylene machubhu ekuchengetera epurasitiki ari nyore kutora nucleic acids (DNA/RNA), zvichikonzera kudzikira kwehuwandu hwenyucleic acid mumhinduro yekuchengetedza.Mukuita, zvinokurudzirwa kushandisa polyethylene-propylene polymer plastiki uye mamwe akanyatsogadziriswa polypropylene mapurasitiki emidziyo kuchengetedza hutachiona nucleic acids.]

(4) Kushanda kwekliniki laboratory hakuna kumira.Sample yekufambisa uye mamiriro ekuchengetera, yakamisikidzwa kushanda kwemakiriniki marabhoritari, dudziro yemhedzisiro uye kutonga kwemhando ndizvo zvinhu zvakakosha kuti uve nechokwadi chechokwadi uye kuvimbika kwemhedzisiro yebvunzo.Zvinoenderana nemhedzisiro yekuongororwa kwemhando yekunze kwakaitwa neClinical Laboratory Center yeNational Health Commission munaKurume 16-24, 2020, pamarabhoritari mazana masere nemakumi mana nemana akagamuchira mhedzisiro, 701 (83.1%) vaive vanokwanisa, uye 143 (16.9%) vaive vasina.Inokodzera, iyo yakazara marabhoritari yekuyedza mamiriro akanaka, asi akasiyana marabhoritari achine mutsauko mukugona kushanda kwevashandi, imwe-inovavarirwa yakanaka yekududzira sampuli kugona, uye kutonga kwemhando.

Nzira yekudzikisa sei nhema isina kunaka yeSARS-CoV-2 nucleic acid yekuona?

Kuderedza zvisizvo zvenhema mu nucleic acid yekuona kunofanirwa kuvandudzwa kubva pamativi mana ekuburitsa manyepo.

(1)Mumasero emunhu ane utachiona mune hutachiwana hwakati.Kuwanda kwehutachiona munzvimbo dzakasiyana dzemuviri wevanofungidzirwa kuti vane hutachiona huchasiyana panguva dzakasiyana.Kana pasina pharynx, inogona kunge iri mubronchial lavage fluid kana tsvina.Kana akawanda marudzi emasampuli anogona kuunganidzwa panguva imwe chete kana pamatanho akasiyana ekufambira mberi kwechirwere kuti aedzwe, Zvinobatsira kudzivirira manyepo.

(2) Masero ane utachiona anofanira kuunganidzwa panguva yekutorwa kwemuenzaniso.Dambudziko iri rinogona kugadziriswa kusvika pamwero mukuru nekusimbisa kudzidziswa kwevatoreri vemuenzaniso.

(3) Yakavimbika IVD reagents.Nekuita tsvakiridzo pamusoro pekuongororwa kwekuita kwekuongorora kwemareagents padanho renyika, uye kukurukura nezvematambudziko aripo, kugona kwekuona kwema reagents kunogona kuvandudzwa uye kunzwisiswa kwekuongorora kunogona kuvandudzwa.

(4) Kushanda kwakarongeka kwemakiriniki marabhoritari.Nekusimbisa kudzidziswa kwevashandi ve laboratori, kuenderera mberi nekuvandudza marabhoritari emhando yekutarisira hurongwa, kuve nechokwadi chekukamukana kwakanaka, uye nekuvandudza kugona kwevashandi kuona, zvinokwanisika kudzikisa zvisizvo zvenhema nekuda kwekutadza kushanda kwerabhoritari.

Zvikonzero zvekuongororwazve kwakanaka kweSARS-CoV-2 nucleic acid bvunzo muvarwere vakapora uye vakaburitswa

Iyo "COVID-19 Diagnosis and Treatment Plan (Trial Seventh Edition)" inotaura zvakajeka kuti imwe yenzira dzekuti varwere veCCIDID-19 varapwe uye vabudiswe muchipatara ndechekuti maviri akateedzana ekufema turakiti samples ane negative nucleic acid bvunzo (angangoita maawa makumi maviri nemana akaparadzana), asi kune vashoma kwazvo Iyo SARS-CoV-2 ye24 nucleic acid yakaongororwazve nekuda kwezvikonzero zvakasiyana-siyana.

(1)SARS-CoV-2 hutachiona hutsva.Izvo zvinodiwa kuti uwedzere kunzwisisa maitiro ayo epathogenic, mufananidzo wakazara wechirwere chinokonzerwa uye maitiro echirwere chekosi.Nokudaro, kune rumwe rutivi, zvakakosha kusimbisa hutungamiri hwevarwere vakasunungurwa uye kuitisa 14-day yekucherechedza kurapwa.Ita kuteedzera, kutarisa kwehutano uye kutungamira kwehutano kudzamisa kunzwisisa kwese chiitiko chechiitiko, kukura uye mhedzisiro yechirwere.

(2)Murwere anogona kutapukirwa neutachiona zvakare.Academician Zhong Nanshan akati: Nekuti varwere vakarapwa vane masoja ekudzivirira chirwere, SARS-CoV-2 inogona kubviswa nemasoja ekudzivirira chirwere paanopinda zvekare.Pane zvikonzero zvakawanda, zvinogona kunge zviri izvo zvakakonzera murwere akapora, kana zvingave zvine chekuita nekuchinja kwehutachiona, kana kunyangwe chikonzero chekuongororwa murabhoritari.Kana iri hutachiona pachahwo, iyo SARS-CoV-2 shanduko inogona kukonzera kuti antibody inogadzirwa nemurwere akapora kusashanda kurwisa hutachiona hwakashandurwa.Kana murwere akatapukirwa nehutachiona hwakashandurwa zvakare, nucleic acid test inogona kuva yakanaka zvakare.

(3) Nezve nzira dzekuedza marabhoritari, nzira yega yega yekuyedza ine painogumira.SARS-CoV-2 nucleic acid yekuona imhaka yesarudzo yekutevedzana kwemajini, kuumbwa kwemareagents, kunzwisiswa kwenzira uye zvimwe zvikonzero, zvinotungamira kumakiti aripo ane yavo yakaderera yekuona miganhu.Mushure mekunge murwere arapwa, utachiona huri mumuviri hunoderera.Kana hutachiwana hwehutachiwana huri mumuenzaniso hunofanira kuongororwa huri pasi pemuganhu wakaderera wekuonekwa, "negative" mhedzisiro ichaonekwa.Zvisinei, chigumisiro ichi hachirevi kuti utachiona huri mumuviri hwakanyangarika zvachose.Utachiona hunogona kunge huri mushure mekumisa kurapwa.Resurgence”, ramba uchikopa.Nokudaro, zvinokurudzirwa kuongorora kamwe chete pavhiki mukati me2 kusvika kumavhiki e4 mushure mekubudiswa.

(4) Nucleic acid ndiyo genetic material yeutachiona.Hutachiona hunouraiwa mushure mekunge murwere arapwa nemishonga inorwisa utachiona, asi zvakasara zvehutachiona zveRNA zvimedu zvichiri kuchengetwa mumuviri wemunhu, uye hazvina kuburitswa zvachose kubva mumuviri.Dzimwe nguva, mune mamwe mamiriro ezvinhu, inogona kuchengetwa zvakanyanya.Nguva yakareba, uye panguva ino iyo nucleic acid test ichave "inononoka" yakanaka.Nekuwedzera kwenguva yekupora kwemurwere, mushure mekunge zvimedu zveRNA zvasara mumuviri zvapera zvishoma nezvishoma, iyo nucleic acid test mhedzisiro inogona kuita yakaipa.

(5) Iyo nucleic acid bvunzo mhedzisiro yeSARS-CoV-2 inongoratidza kuvepo kana kusavapo kwehutachiona RNA, uye haigone kuratidza basa rehutachiona uye kana hutachiona hunotapuriranwa.Izvo zvinodikanwa kuratidza kana murwere ane positive nucleic acid bvunzo zvakare achaita sosi yehutachiona zvakare.Izvo zvinodikanwa kuita hutachiona tsika pamakiriniki masampuli uye kurima hutachiona "mhenyu" kuratidza kuti hunotapukira.

Summary

Muchidimbu, SARS-CoV-2 nucleic acid bvunzo dzenhema dzisina kunaka, retest positives, uye mamwe mamiriro asingaenderane nekuratidzwa kwekiriniki haigone kudzivirirwa zvachose.Mukuongorora chaiko uye kuyedzwa, zvinokurudzirwa kubatanidza zviratidzo zvekiriniki, bvunzo dzekufungidzira (CT) uye kuyedza Labhoritari bvunzo (nucleic acid test + virus-specific antibody bvunzo) inoguma yekuongororwa kwakadzama kudzivirira kupotsa kuongororwa uye kusaziva.Kana mhedzisiro yebvunzo ikawanikwa isingaenderane nekuratidzwa kwekiriniki, zvinokurudzirwa kuita ongororo yakazara yezvese test link (sample muunganidzwa, kutenderera uye kugadzirisa zvinongedzo) kusabvisa SARS-CoV-2 hutachiona hwekutanga kutapukirwa, hutachiona hwekare kana kusanganiswa nehumwe hutachiona hwehutachiona hwekufema, nezvimwe zvinogoneka.Kana mamiriro ezvinhu achibvumidza, zvinokurudzirwa kuunganidza sampuli dzakasimba senge sputum kana alveolar lavage fluid kuti iongororwe zvakare.

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SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)