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Mouse Muswe SuperDirect PCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu yekukurumidza kuburitsa genomic DNA kubva kumakonzo muswe uye mbeva nzeve masampuli ePCR reaction, saka inonyanya kukodzera kune yakakura-chiyero chemazita ekuyedza.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer yapera mukati memaminetsi gumi nemakumi matatu kusvika tembiricha (20-25 ° C). Hapana kupisa kunodiwa, uye mamwe maitiro senge mapuroteni uye RNA kubviswa hazvidiwe. Iyo yakaburitswa trace yakawanda yeDNA inogona kushandiswa seTemplate yePCR reaction.
2 × M-PCR Easy TM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekubudirira uye yakanangana nekusimudzira. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
