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Mouse Muswe Wakananga PCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu yekukurumidza kuburitsa genomic DNA kubva kumakonzo miswe, nzeve, mhasuru uye mamwe matissue sampuli ekuchinja kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65 ° C. Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodikanwa, uye iyo yakaburitswa trace DNA inogona kushandiswa se template yePCR reaction.
2 × M1-PCR Easy TM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe setemplate yekubudirira uye yakanangana nekusimudzira. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
