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Mhuka Tissue Yakananga PCR kit
Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kumhuka dzemuviri masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65°C. Hapana mamwe maitiro senge mapuroteni uye RNA kubviswa anodikanwa, uye iyo yakasunungurwa trace DNA inogona kushandiswa se template yePCR reaction.
2× PCR NyoreTM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekushanda uye kwakasarudzika. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Mouse Muswe SuperDirect PCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu yekukurumidza kuburitsa genomic DNA kubva kumakonzo muswe uye mbeva nzeve masampuli ePCR reaction, saka inonyanya kukodzera kune yakakura-chiyero chemazita ekuyedza.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer yapera mukati memaminetsi gumi nemakumi matatu kusvika tembiricha (20-25 ° C). Hapana kupisa kunodiwa, uye mamwe maitiro senge mapuroteni uye RNA kubviswa hazvidiwe. Iyo yakaburitswa trace yakawanda yeDNA inogona kushandiswa seTemplate yePCR reaction.
2 × M-PCR Easy TM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekubudirira uye yakanangana nekusimudzira. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Mhuka Tissue Yakananga PCR kit-UNG
Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kumhuka dzemuviri masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65°C. Hapana mamwe maitiro senge mapuroteni uye RNA kubviswa anodikanwa, uye iyo yakasunungurwa trace DNA inogona kushandiswa se template yePCR reaction.
2×PCR NyoreTM Sanganisa (UNG) inoshandisa dUTP pachinzvimbo cheDTTP pahwaro hwe2×PCR NyoreTM Sanganisa, uye unowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kudzikisira template ine DUTP panguva imwe chete. Pamberi pePCR kuita, iyo enzyme yeUNG inoshandiswa kudzikisira iyo PCR chigadzirwa chine uracil. Iyo enzyme yeUNG haizove nechero mhedzisiro patemplate iyo isina uracil, nekudaro ichivimbisa huchokwadi uye kurongeka kwekusimudzira nekudzivirira mukana wePCR zvigadzirwa zvinosvibisa panguva yakakura yekuyera kwemajini.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Cell Yakananga RT qPCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer system iyo inogona kukurumidza kuburitsa RNA kubva kune yakakwenenzverwa maseru sampuli yeRT-qPCR maitiro, nokudaro ichibvisa iyo inopedza nguva uye inorema RNA yekuchenesa maitiro. Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete. Iyo 5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR reagents yakapihwa nekiti inogona kukurumidza uye kunyatso kuwana chaiyo-nguva yakawanda huwandu hwePCR mhedzisiro.
5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR ine yakasimba inhibitor shiviriro, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga. Iyi kit ine yakasarudzika RNA yakakwira-kuyananisa Foregene reverse transcriptase, uye Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
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Mouse Muswe Wakananga PCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu yekukurumidza kuburitsa genomic DNA kubva kumakonzo miswe, nzeve, mhasuru uye mamwe matissue sampuli ekuchinja kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65 ° C. Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodikanwa, uye iyo yakaburitswa trace DNA inogona kushandiswa se template yePCR reaction.
2 × M1-PCR Easy TM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe setemplate yekubudirira uye yakanangana nekusimudzira. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Mouse Muswe Wakananga PCR Kit-UNG
Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu yekukurumidza kuburitsa genomic DNA kubva kumakonzo miswe, nzeve, mhasuru uye mamwe matissue sampuli ekuchinja kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65 ° C. Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodikanwa, uye iyo yakaburitswa trace DNA inogona kushandiswa se template yePCR reaction.
2 × M1-PCR Easy TM Sanganisa (UNG) inoshandisa dUTP pachinzvimbo cheDTTP pahwaro hwe2 × M1-PCR Easy TM Mix (UNG), uye inowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kudzikisira template ine DUTP pa nguva imwechete. Pamberi pePCR kuita, iyo enzyme yeUNG inoshandiswa kudzikisira iyo PCR chigadzirwa chine uracil. Iyo enzyme yeUNG haizove nemhedzisiro patemplate iyo isina uracil, nokudaro ichisimbisa iyo chaiyo uye kurongeka kwekusimudzira uye kudzivirira mukana wekuyera kukuru kwemajini. Kusvibiswa kwezvigadzirwa zvePCR zvakaitika.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Zebra Hove Yakananga PCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kune zebrafish uye mamwe emvura matsva ehove matishu, muswe mapini kana hove mazai masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini. Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65 ° C. Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodikanwa, uye iyo yakaburitswa trace DNA inogona kushandiswa se template yePCR reaction.
2 × PCR Easy TM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekubudirira uye kwakasarudzika. Iyi reagent ine ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer. Inoshandiswa pamwe chete neiyo lysis buffer inogona kukurumidza uye nyore kuona masampuli, uye ine hunhu hwekunzwisisa kwakanyanya, kusimba kwakasimba, uye kugadzikana kwakanaka.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Mbizi Hove Yakananga PCR Kit-UNG
Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kune zebrafish uye mamwe emvura matsva ehove matishu, muswe mapini kana hove mazai masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini. Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65 ° C. Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodikanwa, uye iyo yakaburitswa trace DNA inogona kushandiswa se template yePCR reaction.
2 × PCR Easy TM Sanganisa (UNG) inoshandisa dUTP pachinzvimbo cheDTTP pahwaro hwe2 × PCR Easy TM Sanganisa, uye inowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kudzikisira template ine DUTP panguva imwe chete. Pamberi pePCR kuita, iyo enzyme yeUNG inoshandiswa kudzikisira iyo PCR chigadzirwa chine uracil. Iyo enzyme yeUNG haizove nemhedzisiro patemplate iyo isina uracil, nokudaro ichisimbisa iyo chaiyo uye kurongeka kwekusimudzira uye kudzivirira mukana wekuyera kukuru kwemajini. Kusvibiswa kwezvigadzirwa zvePCR zvakaitika.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
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Dyara shizha Direct PCR Plus kit
Ichi chigadzirwa chinoshandisa yakasarudzika lysis buffer system ku lyse mashizha emashizha. Iyo lysate inogona kushandiswa se template isina kunatswa. Mushure mekuwedzera iwo ekutanga, iyo PCR Sanganisa yeiyi kit inogona kushandiswa mukusimudzira.
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Dyara Mbeu Yakananga PCR Kit I / II
Iyi kit inoshandisa yakasarudzika lysis buffer system ku lyse mbeu yekudyara. Iyo lysate inogona kushandiswa se template isina kunatswa. Mushure mekuwedzera iwo ekutanga, iyo PCR Sanganisa yeiyi kit inogona kushandiswa mukusimudzira.
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Dyara shizha Direct PCR pamwe kit-UNG
Kubva paPlant Leaf Direct PCR Plus kit, dUTP inoshandiswa panzvimbo yeDTTP, uye iyo enzyme yeUNG inogona kudzikisira template ine DUTP inowedzerwa. Nenzira iyi, iko kusarongeka uye kurongeka kwekusimudzira kunovimbiswa, uye dambudziko rekusvibiswa kwechigadzirwa chePCR chinogona kuitika panguva yekuyedzwa kwemageneti kwakadzivirirwa.
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Dyara shizha yakananga PCR kit-UNG
Kubva paPlant Leaf Direct PCR kit, dUTP inoshandiswa panzvimbo yeDTTP, uye iyo enzyme yeUNG inogona kudzikisira template ine DUTP inowedzerwa. Nenzira iyi, iko kusarongeka uye kurongeka kwekusimudzira kunovimbiswa, uye dambudziko rekusvibiswa kwechigadzirwa chePCR chinogona kuitika panguva yekuyedzwa kwemageneti kwakadzivirirwa.
