-
Cell Yakananga RT qPCR Kit
Iyi kit inoshandisa yakasarudzika lysis buffer system iyo inogona kukurumidza kuburitsa RNA kubva kune yakakwenenzverwa maseru sampuli yeRT-qPCR maitiro, nokudaro ichibvisa iyo inopedza nguva uye inorema RNA yekuchenesa maitiro. Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete. Iyo 5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR reagents yakapihwa nekiti inogona kukurumidza uye kunyatso kuwana chaiyo-nguva yakawanda huwandu hwePCR mhedzisiro.
5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR ine yakasimba inhibitor shiviriro, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga. Iyi kit ine yakasarudzika RNA yakakwira-kuyananisa Foregene reverse transcriptase, uye Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
