Ropa DNA Midi Kit (1-5ml) Ropa Isolation Midi Kits yeDNA kubva muRopa
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva kune anticoagulated ropa (1-5ml).
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Muenzaniso weropa une nhamba huru yemasero matsvuku eropa uye masero eplatelet, izvo zvinoita kuti kushandiswa kweropa kuwedzere kunetseka.Nekudaro, iyo genomic DNA yeropa yakatorwa neyakajairwa nzira haina kukwirira mukuchena uye zvirimo zvakaderera.Kiti iyi inoshandisa iyo nyowani Foregene Protease Plus uye yakasarudzika BL1 uye BL2 buffer masisitimu, anogona kugaya zvachose ropa rinopesana neropa uye.coagulationmasampuli munguva pfupi pfupi, nekudaro kudzivirira kuderedzwa kweDNA kusvika pamwero mukuru uye kuwana huwandu hukuru hwegenomic DNA.Iyo inokurumidza kugadzirisa ropa system uye yakapusa spin column mashandiro anorerutsa zvakanyanya kutorwa kweropa genome, kuitira kuti yemhando yepamusoro, yakakwirira-kuchena genomic DNA inogona kuwanikwa mukati memaminitsi makumi mana.
Iyo DNA-chete silica gel membrane inoshandiswa mu spin column yekiti iyi chinhu chitsva chakasarudzika chekambani, chinonyatso uye chinoshambadza DNA, uye chinogona kubvisa RNA, mapuroteni ekusachena, ion uye mamwe maoga emakemikari mumaseru kusvika pamwero mukuru.Zvimedu zveDNA zvakawanikwa zvakakura, zvine kuchena kwepamusoro, kugadzikana uye kuvimbika kwemhando, uye huwandu hwekutakura huwandu hwe spin column ndeye 80 μg DNA.Iyo DNA inowanikwa inogona kushandiswa kune mamolecular biology kuyedza senge kudzora kugaya, PCR, Southern hybridization, kuvaka raibhurari uye zvichingodaro.Iyo kit inogona kugadzira kusvika ku5ml yeropa panguva kuti iwane huwandu hwakakwana hwepamusoro-kuchena genomic DNA.
Zvinotsanangurwa
50 Preps, 100 Preps, 250 Preps
Kit zvikamu
| Buffer BL1 |
| Buffer BL2* |
| Buffer DC |
| Buffer PW* |
| Buffer WB1 |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maprotease akafanana, uye inogaya sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.
-Yakanaka: Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4°C pasi-tembiricha centrifugation kana ethanol kunaya kweDNA.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando anticoagulated ropa rose.
Workflow
Storage uye Sherufu upenyu
-Iyi kit inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pane tembiricha yekamuri (15-25℃);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8℃.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease Plus mhinduro ine chimiro chakasiyana-siyana, iyo inoshanda kana yakachengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu), basa rayo uye kugadzikana kuchava nani kana yakachengetwa pa4 ° C, saka inokurudzirwa kuichengeta pa4 ° C, yeuka kuti usaichengeta pa -20℃.
