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Mhuka Tissue Yakananga PCR kit
Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kumhuka dzemuviri masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.
Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65°C. Hapana mamwe maitiro senge mapuroteni uye RNA kubviswa anodikanwa, uye iyo yakasunungurwa trace DNA inogona kushandiswa se template yePCR reaction.
2× PCR NyoreTM Sanganisa ine kushivirira kwakasimba kune PCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe se template yekushanda uye kwakasarudzika. Iyi reagent ine Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.
D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.
