Mhuka Tissue Yakananga PCR Kit-UNG

  • Animal Tissue Direct PCR kit-UNG

    Mhuka Tissue Yakananga PCR kit-UNG

    Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kumhuka dzemuviri masampuli ekushanduka kwePCR, saka inonyanya kukodzera kuyerwa kukuru kwemajini.

    Maitiro ekuburitsa genomic DNA kubva kune lysis buffer inopedzwa mukati memaminitsi 10-30 pa65°C. Hapana mamwe maitiro senge mapuroteni uye RNA kubviswa anodikanwa, uye iyo yakasunungurwa trace DNA inogona kushandiswa se template yePCR reaction.

    2×PCR NyoreTM Sanganisa (UNG) inoshandisa dUTP pachinzvimbo cheDTTP pahwaro hwe2×PCR NyoreTM Sanganisa, uye unowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kudzikisira template ine DUTP panguva imwe chete. Pamberi pePCR kuita, iyo enzyme yeUNG inoshandiswa kudzikisira iyo PCR chigadzirwa chine uracil. Iyo enzyme yeUNG haizove nechero mhedzisiro patemplate iyo isina uracil, nekudaro ichivimbisa huchokwadi uye kurongeka kwekusimudzira nekudzivirira mukana wePCR zvigadzirwa zvinosvibisa panguva yakakura yekuyera kwemajini.

    D-Taq DNA polymerase iDNA polymerase inonyanya kugadzirwa naForegene kune yakananga PCR maitiro. D-Taq DNA polymerase ine kushivirira kwakasimba kune dzakasiyana siyana dzePCR reaction inhibitors, uye inogona kunyatsoita huwandu hweDNA mune akasiyana maitiro ekugadzirisa masisitimu, uye iyo yekumhanyisa yekumhanyisa inogona kusvika 2Kb / min, iyo inonyanya kukodzera Direct PCR kuita.