Iwo maDNA mamaki anogadzirwa nekambani yedu ese anowanikwa ne enzyme digestion yeplasmids.Iwo mamaki anogadzirwa neichi maitiro ane akachena kumashure, akajeka mabhendi, yakagadzikana mhando uye anogona kuwana chaiyo quantification yemaki.Chigadzirwa chacho chine madhayi maviri (cyan dye uye yellow), uye chiyero chekufamba kwe electrophoresis chinogona kutongwa nekushanduka kwemavara panguva ye electrophoresis.Nhamba yekufamba kweiyo cyan dye yakafanana neye 3-5kb mu 1% agarose gel, uye chiyero chekufamba kweyero dhayi ndeyeNyero yekufambisa yakafanana neyeye 50bp band, uye kufambira mberi kwe electrophoresis kunogona kuonekwa zvakananga neziso rakashama.Zviri nyore kushandisa uye mufananidzo we electrophoresis wakajeka.

Ichi chigadzirwa chigadzirwa chakagadzirira-kushandisa uye chatova ne1x Inorodha Buffer.Iwe unogona kutora zvakananga huwandu hwakakodzera hweMucherechedzo we electrophoresis zvinoenderana nekuyedza zvinodiwa.BM5000 DNA Marker ine 8 DNA mabhendi, mabhandi eDNA ndeaya: 100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000)ng0/0b/5μl, 1000)ng0/5/5p(l) ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).